A simple modification of the Baermann method for diagnosis of strongyloidiasis

Memórias do Instituto Oswaldo Cruz

Francisco Hernández-chavarría¹  Leticia Avendaño¹ 

[1] ,Universidad de Costa Rica Facultad de Microbiología Unidad de Microscopia ElectrónicaSan José,Costa Rica

关键词: Strongyloides stercoralis;    Baermann method;    agar culture plate method;    direct smear;    intestinal parasites;   

DOI  :  10.1590/S0074-02762001000600011

来源: SciELO

【 摘 要 】

The diagnosis of Strongyloides stercoralis infections is routinely made by microscopic observation of larvae in stool samples, a low sensitivity method, or by other, most effective methods, such as the Baermann or agar culture plate methods. We propose in this paper a practical modification of Baermann method. One hundred and six stool samples from alcoholic patients were analyzed using the direct smear test, agar culture plate method, the standard Baermann method, and its proposed modification. For this modification the funnel used in the original version of the method is substituted by a test tube with a rubber stopper, perforated to allow insertion of a pipette tip. The tube with a fecal suspension is inverted over another tube containing 6 ml of saline solution and incubated at 37°C for at least 2 h. The saline solution from the second tube is centrifuged and the pellet is observed microscopically. Larva of S. stercoralis were detected in six samples (5.7%) by the two versions of the Baermann method. Five samples were positive using the agar culture plate method, and only in two samples the larva were observed using direct microscopic observation of fecal smears. Cysts of Endolimax nana and Entamoeba histolytica/dyspar were also detected in the modification of Baermann method. Data obtained by the modified Baermann method suggest that this methodology may helps concentrate larvae of S. stercoralis as efficiently as the original method.

【 授权许可】

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