期刊论文详细信息
Memórias do Instituto Oswaldo Cruz
Changes in nuclear phenotypes following cold shock in Panstrongylus megistus (Burmeister)
Simone L Garcia2  Maria Luiza S Mello2  Nancy L Garcia1  Vera Lúcia Cc Rodrigues1 
[1] ,Universidade Estadual de Campinas (Unicamp) Instituto de Biologia Departamentos de Biologia Celular e Parasitologia
关键词: Panstrongylus megistus;    cold shock;    nuclear phenotypes;    cell survival;    cell death;   
DOI  :  10.1590/S0074-02762000000600027
来源: SciELO
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【 摘 要 】

The nuclear phenotypes of Malpighian tubule epithelial cells of 5th instar male nymphs of the blood-sucking insect Panstrongylus megistus were studied immediately after a short (1 h) cold shock at 0ºC, and 10 and 30 days later. The objective was to compare the responses to a cold shock with those known to occur after hyperthermia in order to provide insight into the cellular effect of cold in this species. Nuclei which usually exhibited a conspicuous Y chromosome chromocenter were the most frequent phenotype in control and treated specimens. Phenotypes in which the heterochromatin was unravelled, or in which there was nuclear fusion or cell death were more abundant in the shocked specimens. Most of the changes detected have also been found in heat-shocked nymphs, except for nuclear fusion which generates giant nuclei and which appeared to be less effective or necessary than that elicited after heat shock. Since other studies showed that a short cold shock does not affect the survival of more than 14% of 5th instar nymphs of P. megistus with domestic habit and can induce tolerance to a prolonged cold shock, heat shock proteins proteins are probably the best candidates for effective protection of the cells and the insects from drastic damage caused by low temperature shocks.

【 授权许可】

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