Mexican Trypanosoma cruzi stocks: analysis of minicircle kDNA homologies by cross-hybridization

Memórias do Instituto Oswaldo Cruz

Marie-france Bosseno²  Bertha Espinoza¹  Beatriz Sánchez¹  Simone Frédérique Brenière¹ 

[1] ,UMR CNRS Montpellier,France

关键词: Trypanosoma cruzi;    Mexico;    kDNA minicircle;   

DOI  :  10.1590/S0074-02762000000400005

来源: SciELO

【 摘 要 】

Homologies of minicircle kDNA of 27 Mexican stocks were studied by cross-hybridization with four kDNA probes derived from three reference stocks belonging to groups Trypanosoma cruzi I (SO34 cl4 and Silvio) and T. cruzi II (MN) and one Mexican stock. High homologies were only observed with Silvio (six stocks) and Mexican probes (11 stocks). After 30 min exposure (low homology) additional stocks were recognized with SO34 cl4 (three stocks) and Silvio (six stocks) probes; with the Mexican probe only five stocks remained non-reactive. All the stocks were typed by isoenzyme (16 loci) and Mexican stocks belonged to T. cruzi I. Hybridization patterns were not strictly correlated with the observed clustering and cross-hybridization of kDNA minicircles is not available to distinct Mexican stocks.

【 授权许可】

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