Direct methods for detecting picorna-like virus from dead and alive Triatomine insects

Memórias do Instituto Oswaldo Cruz

Gs Rozas Dennis²  Jl La Torre¹  Oa Muscio¹  Dma Guérin¹ 

[1] ,Centro de Recursos Renovables de la Zona SemiáridaBahía Blanca,Argentina

关键词: picorna-like virus;    Triatoma virus;    Triatoma infestans;    transmission electron microscope methods;   

DOI  :  10.1590/S0074-02762000000300006

来源: SciELO

【 摘 要 】

In this work we report four different destructive and non-destructive methods for detecting picorna-like virus particles in triatomines. The methods are based on direct observation under transmission electron microscope and they consist of four ways to prepare samples of presumable infected material. The samples are prepared processing dead or alive insect parts, or even dry or fresh insect feces. The methods can be used as analytical or preparative techniques, for quantifying virus infection and checking virus integrity as well. In this work the four methods are applied in order to detect Triatoma virus (TrV) particles in T. infestans colonies.

【 授权许可】

CC BY   
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