Antigenic and Genetic Characterization of Twenty-six Strains of Human Respiratory Syncytial Virus (Subgroup A) Isolated During Three Consecutive Outbreaks in Havana City, Cuba

Memórias do Instituto Oswaldo Cruz

Angel Valdivia²  Grehete González²  Danay Chacón²  Clara Savón²  Anselmo Otero²  Odalys Váldes²  Reynel Cancio²  Suset Oropesa²  José A Melero¹  Blanca García-barreno¹  Angel Goyenechea² 

[1] ,Instituto de Medicina Tropical 'Pedro Kouri'La Habana,Cuba

关键词: respiratory syncytial virus;    molecular characterization;    restriction mapping;    monoclonals antibodies;   

DOI  :  10.1590/S0074-02761999000400007

来源: SciELO

【 摘 要 】

Twenty-six human respiratory syncytial virus strains (subgroup A) isolated from three outbreaks in Havana City during the period 1994/95, 1995/96 and 1996/97 were analyzed to determine their antigenic and genetic relationships. Analyses were performed by monoclonal antibodies and restriction mapping (N gene) following amplification of the select region of the virus genome by polymerase chain reaction. All isolated strains were classified as subgroup A by monoclonal antibodies and they showed a restriction pattern NP4 that belonged to subgroup A. Thus the results obtained in this work, showed a close relation (100%) between antigenic and genetic characterization of the isolated strains in our laboratory. These methods permit the examination of large numbers of isolates by molecular techniques, simplifying the researchs into the molecular epidemiology of the virus.

【 授权许可】

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