Programmed Cell Death in Procyclic Form Trypanosoma brucei rhodesiense - Identification of Differentially Expressed Genes during Con A Induced Death

Memórias do Instituto Oswaldo Cruz

Susan C Welburn¹  Simon Lillico²  Noel B Murphy² 

[1] ,University of Edinburgh,Scotland

关键词: Trypanosoma brucei rhodesiense;    programmed cell death;    apoptosis;    differential gene expression;    proto-apoptosis;   

DOI  :  10.1590/S0074-02761999000200020

来源: SciELO

【 摘 要 】

Trypanosoma brucei rhodesiense can be induced to undergo apoptosis after stimulation with Con A. As cell death in these parasites is associated with de novo gene expression we have applied a differential display technique, Randomly Amplified Differential Expressed Sequence-Polymerase Chain Reaction (RADES-PCR) to the study of gene expression during Con A induced cell death in these organisms. Twenty-two differentially displayed products have been cloned and sequenced. These represent the first endogenous genes to be identified as implicated in cellular death in trypanosomatids (the most primitive eukaryote in which apoptosis has been described). Evidence for an ancestral death machinery, `proto-apoptosis' in single celled organisms is discussed.

【 授权许可】

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