Immunization with PIII, a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, affects nitric oxide production by murine spleen cells

Memórias do Instituto Oswaldo Cruz

Diana Magalhães De Oliveira¹  Silvânia Antunes Do Carmo¹  David N Silva-teixeira¹  Alfredo Miranda Goes¹ 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de Bioquímica e ImunologiaBelo Horizonte MG ,Brasil

关键词: Schistosoma mansoni;    immunization;    PIII;    nitric oxide;    in vitro granuloma;   

DOI  :  10.1590/S0074-02761998000700028

来源: SciELO

【 摘 要 】

Nitric oxide (NO) is an important effector molecule involved in immune regulation and defense. NO produced by cytokine-activated macrophages was reported to be cytotoxic against the helminth Schistosoma mansoni. Identification and characterization of S. mansoni antigens that can provide protective immunity is crucial for understanding the complex immunoregulatory events that modulate the immune response in schistosomiasis. It is, then, essential to have available defined, purified parasite antigens. Previous work by our laboratory identified a fraction of S. mansoni soluble adult worm antigenic preparation (SWAP), named PIII, able to elicit significant in vitro cell proliferation and at the same time lower in vitro and in vivo granuloma formation when compared either to SEA (soluble egg antigen) or to SWAP. In the present work we report the effect of different in vivo trials with mice on their spleen cells ability to produce NO. We demonstrate that PIII-immunization is able to significantly increase NO production by spleen cells after in vitro stimulation with LPS. These data suggest a possible role for NO on the protective immunity induced by PIII.

【 授权许可】

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