The Amazonia Variant of Vibrio cholerae: Molecular Identification and Study of Virulence Genes

Memórias do Instituto Oswaldo Cruz

Mas Baptista²  Jrc Andrade¹  Acp Vicente¹  Ca Salles¹  A Coelho¹ 

[1] ,Universidade Federal do Rio de Janeiro

关键词: Vibrio cholerae;    Amazonia;    toxR;    outer membrane proteins;    protease;    Lon;   

DOI  :  10.1590/S0074-02761998000500008

来源: SciELO

【 摘 要 】

The pathogenic O1 Amazonia variant of Vibrio cholerae has been shown previously to have a cytotoxin acting on cultured Vero and Y-1 cells, and to lack important virulence factors such as the cholera toxin (Coelho et al. 1995a). This study extends the molecular analysis of the Amazonia strains, detecting the presence of the toxR gene, with a very similar sequence to that of the El Tor and classical biotypes. The outer membrane proteins are analyzed, detecting a variation among the group of Amazonia strains, with three different patterns found. As a by-product of this work a polymerase chain reaction fragment was sequenced, reading part of the sequence of the Lon protease of the Amazonia strains. This gene was not previously described in V. cholerae, but its sequence is present in the TIGR database specific for this species.

【 授权许可】

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