The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins

Memórias do Instituto Oswaldo Cruz

Márcio Viana Ramos²  Renato De Azevedo Moreira²  José Tadeu Abreu Oliveira²  Benildo Sousa Cavada²  Pierre Rougé¹ 

[1] ,Universidade Federal do Ceará Departamento de Bioquímica e Biologia Molecular Laboratório de LectinasFortaleza CE ,Brasil

关键词: Canavalia maritima;    carbohydrate-binding specificity;    Diocleinae;    Dioclea grandiflora;    lectins;    molecular modelling;   

DOI  :  10.1590/S0074-02761996000600021

来源: SciELO

【 摘 要 】

The carbohydrate-binding specificity of lectins from the seeds of Canavalia maritima and Dioclea grandiflora was studied by hapten-inhibition of haemagglutination using various sugars and sugar derivatives as inhibitors, including N-acetylneuraminic acid and N-acetylmuramic acid. Despite some discrepancies, both lectins exhibited a very similar carbohydrate-binding specificity as previously reported for other lectins from Diocleinae (tribe Phaseoleae, sub-tribe Diocleinae). Accordingly, both lectins exhibited almost identical hydropathic profiles and their three-dimensional models built up from the atomic coordinates of ConA looked very similar. However, docking experiments of glucose and mannose in their monosaccharide-binding sites, by comparison with the ConA-mannose complex used as a model, revealed conformational changes in side chains of the amino acid residues involved in the binding of monosaccharides. These results fully agree with crystallographic data showing that binding of specific ligands to ConA requires conformational chances of its monosaccharide-binding site.

【 授权许可】

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