Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs

Memórias do Instituto Oswaldo Cruz

Sj Semião-santos²  P Abranches¹  Mcd Silva-pereira¹  Gm Santos-gomes¹  Jp Fernandes¹  Jcm Vetter² 

[1] ,University of Amsterdam Department of Medical Microbiology Laboratory of ParasitologyAmsterdam,The Netherlands

关键词: canine;    vulpine;    leishmaniasis;    direct agglutination test;    immunofluorescence technique;   

DOI  :  10.1590/S0074-02761996000600018

来源: SciELO

【 摘 要 】

A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres >1:320 and >1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence of L. infantum infection in the canine and vulpine populations appear to be of high magnitude.

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