N-terminal amino acid sequences of the major outer membrane proteins from a Neisseria meningitidis group B strain isolated in Brazil

Memórias do Instituto Oswaldo Cruz

Salvatore Giovanni De Simone²  Solange Af Soares¹  Andre La Souza¹  Maria Gm Danelli¹ 

[1] ,Instituto Oswaldo Cruz Departamento de Bioquímica e Biologia Molecular Laboratório de Microseqüenciamento de Proteínas

关键词: Neisseria meningitidis group B;    outer membrane proteins;    class specific proteins;    N-terminal amino acid sequence;   

DOI  :  10.1590/S0074-02761996000100020

来源: SciELO

【 摘 要 】

The four dominant outer membrane proteins (46, 38, 33 and 28 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in a semi-purified preparation of vesicle membranes of a Neisseria meningitidis (N44/89, B:4:P1.15:P5.5,7) strain isolated in Brazil. The N-terminal amino acid sequence for the 46 kDa and 28 kDa proteins matched that reported by others for class 1 and 5 proteins respectively, whereas the sequence (25 amino acids) for the 38 kDa (class 3) protein was similar to class 1 meningococcal proteins. The sequence for the 33 kDa (class 4) was unique and not homologous to any known protein.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License