期刊论文详细信息
Memórias do Instituto Oswaldo Cruz | |
Identification of species related to Anopheles (Nyssorhynchus) albitarsis by random amplified polymorphic DNA-Polymerase chain reaction (Diptera: Culicidae) | |
Richard C. Wilkerson1  Thomas V. Gaffigan1  José Bento Lima2  | |
[1],Walter Reed Army Institute of ResearchWashington,USA | |
关键词: Anopheles (Nyssorhynchus) albitarsis; Anopheles (Nyssorhynchus) marajoara; Anopheles (Nyssorhynchus) deaneaorum; RAPD PCR; identification; | |
DOI : 10.1590/S0074-02761995000600013 | |
来源: SciELO | |
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【 摘 要 】
Species-specific Random Amplified Polymorphic DNA-Polymerase chain Reaction (RAPD-PCR) markers were used to identify four species related to Anopheles (Nyssorhynchus) albitarsis Lynch-Arribàlzaga from 12 sites in Brazil and 4 in Venezuela. In a previous study (Wilkerson et al. 1995), which included sites in Paraguay and Argentina, these four species were designated "A", "B", "C" and "D". It was hypothesized that species A is An. (Nys.) albitarsis, species B is undescribed, species C is An. (Nys) marajoara Galvão and Damasceno and species D is An. (Nys.) deaneorum Rosa-Freitas. Species D, previously characterized by RAPD-PCR from a small sample from northern Argentina and southern Brazil, is reported here from the type locality of An. (Nys.) deaneorum, Guajará-Mirim, state of Rondônia, Brazil. Species C and D were found by RAPD-PCR to be sympatric at Costa Marques, state of Rondônia, Brazil. Species A and C have yet to be encountered at the same locality. The RAPD markers for species C were found to be conserved over 4,620 km; from Iguape, state of São Paulo, Brazil to rio Socuavo, state of Zulia, Venezuela. RAPD-PCR was determined to be an effective means for the identification of unknown species within this species complex.【 授权许可】
CC BY
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