Initiation of primary cell cultures from embryos of the mosquitoes Anopheles albimanus and Aedes taeniorhynchus (Diptera: Culicidae)

Memórias do Instituto Oswaldo Cruz

Felio J. Bello García²  Jorge Boshell¹  Gloria Rey¹  Alberto Morales¹  Victor Alberto Olano¹ 

[1] ,Universidad de La Salle Departamento de Biologia y Quimica Santafé de Bogotá,Colombia

关键词: mosquitoes;    cell cultures;    culture media;    karyotype;   

DOI  :  10.1590/S0074-02761995000400024

来源: SciELO

【 摘 要 】

Primary cell cultures were obtained from eggs of Anopheles albimanus and Aedes taeniorhynchus mosquitoes, vectors of human malaria and of Venezuelan equine encephalitis virus, respectively. The cellular growth of the An. albimanus cells began four weeks after explanting the embryonic tissues in MK/VP12 medium, supplemented with 15% fetal bovine serum. The culture showed heterogeneous cellular morphology. With regard to the Ae. taeniorhynchus culture, growth occurred three weeks after initiating the culture in MM/VP12 medium. The majority of cells were small and round. Karyotypes were examined in the latter species.

【 授权许可】

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