Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey

Memórias do Instituto Oswaldo Cruz

Juan A. Ramos²  Jesus A. Alvarez²  Julio V. Figueroa¹  Jose Solis²  Roger I. Rodriguez¹  Ruben Hernandez²  Gerald M. Buening¹  Carlos A. Vega² 

[1] ,INIFAP Cenid-Parasitologia Unidad de HemoprotozoariosMorelos,Mexico

关键词: Babesia bigemina;    DNA probe;    Epidemiology;    IFAT;   

DOI  :  10.1590/S0074-02761992000700035

来源: SciELO

【 摘 要 】

An epidemiological survey was conducted in south east Mexico, in an effort to establish the serological reactivity and carrier status to Babesia bigemina of an indigenous cattle population. The prevalance was obtained through the Indirect Fluorescent Antibody Test (IFAT), using an in vitro culture-derived B. bigemina antigen. A specific, digoxigenin-coupled, ~6kb B. bigemina-DNA probe (BBDP), was used to indicate the presence of the parasite. Serum samples from 925 animals of all ages, were obtained within the three regions (I, II, III) of the state of Yucatan and tested by IFAT. In addition, whole blood samples draw from 136 of the same animals of region II were analyzed using the BBDP. Positive IFAT (IFAT+) reactions were observed in 531 sera for a 57% overall prevalence. Regional values were: I = 157 + (56%), II = 266 + (68%) and III = 108 + (42%). Only 32 (23%) of the blood samples tested with BBDP showed distinctive hybridization signal, in contrast with 100 (73%) IFAT + animals. The responses distribution for IFAT vs. BBDP was: +/+ 23, +/- 77, -/+ 9 and -/- 27 respectively. It was found that the analytical sinsitivity of BBDP appears to be low for its utilization is widespread epidemiological surveys. It was considered, however, that the colorimetric probe mifht to be useful to safely detect transmission prone carriers, since it is able to detect parasitemias as low as 0.001%.

【 授权许可】

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