Brazilian dengue virus type 1 replication in mosquito cell cultures

Memórias do Instituto Oswaldo Cruz

Ortrud Monika Barth¹  Hermann G. Schatzmayr¹ 

[1] ,Instituto Oswaldo Cruz Departamento de Virologia Rio de Janeiro,Brasil

关键词: Flaviviridae;    dengue-1;    virus-replication;    ultrastructure;   

DOI  :  10.1590/S0074-02761992000100001

来源: SciELO

【 摘 要 】

The development of dengue viruses type 1 obtained from accute human sera and inoculated into mosquito cell cultures, was observed by standard transmission electron microscopy and cytochemical staining. It follows the trans-type mechanism already estabilished of other dengue types. Directed passage of single virus particles across the cell membrane seems to be a pathway of entry and exit in dengue-1 infected cells. The nature of numerous electron translucent vesicles and tubules, produced simmultaneously during virus replication inside the rough endoplasmic reticulum, was analyzed by cytochemical tests. The largest amount of virus particles was produced inside cell syncytia.

【 授权许可】

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