Proteinase inhibitors in Brazilian leguminosae

Memórias do Instituto Oswaldo Cruz

C. A. M. Sampaio²  M. L. V. Oliva¹  A. S. Tanaka²  M. U. Sampaio² 

[1] ,Escola Paulista de Medicina Departamento de Bioquímica São Paulo,Brasil

关键词: Bauhinia bauhinioides;    Bauhinia pentandra;    coagulation;    Enterolobium contortisiliquum;    proteinase inhibitor;    Torresea cearensis;   

DOI  :  10.1590/S0074-02761991000600047

来源: SciELO

【 摘 要 】

Serine proteinase inhitors, in the seeds of several Leguminosae from the Pantanal region (West Brazil), were studied using bovine trypsin, a digestive enzyme, Factor XIIa and human plasma Kallikrein, two blood clotting factors. The inhibitors were purified from Enterolobium contortisiliquum (Mr=23,000), Torresea cearensis (Mr = 13,000), Bauhinia pentandra (Mr = 20,000) and Bauhinia bauhinioides (Mr = 20,000). E. contortisiliquum inhibitor inactivates all three enzymes, whereas the T. cearensis inhibitor inactivates trypsin and Factor XSSa, but does nor affect plasma kallikrein; both Bauhinia inhibitors, on the other hand, inactivate trypsin and plasma kallikrein but only the Bpentandra inhibitor affects Factor XIIa. Ki values were calculated between 10 [raised to the power of] -7 and 10 [raised to the power of] -8 M.

【 授权许可】

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