Molecular analysis of yellow fever virus 17DD vaccine strain

Memórias do Instituto Oswaldo Cruz

Paulo R. Post²  Claudia N. D. Santos²  Ricardo Carvalho¹  Oscar S. Lopes¹  Ricardo Galler² 

[1] ,Instituto Oswaldo Cruz Departamento de Bioquímica e Biologia Molecular Rio de Janeiro,Brasil

关键词: yellow fever;    vaccine;    17DD strain;    envelope;    RNA;   

DOI  :  10.1590/S0074-02761991000200015

来源: SciELO

【 摘 要 】

The Oswaldo Cruz Foundation produces most of the yellow fever (YF) vaccine prepared world wide. As part of a broader approach to determine the genetic variability in YF l7D seeds and vaccines and its relevance to viral attenuation the 17DD virus was purifed directly from chick embryo homogenates which is the source of virus used for vaccination of millions of people in Brazil and other countries for half a century. Neutralization and hemagglutination tests showed that the purified virus is similar to the original stock. Furthermore, radioimmune precipitation of 35S-methionine-labeled viral proteins using mouse hyperimmune ascitic fluid revealed identical patterns for the purified 17DD virus and the YF l7D-204 strain except for the 17DD E protein which migrated slower on SDS-PAGE. This difference is likely to be due to N-linked glycosylation. Finally, comparison by northern blot nybridization of virion RNAs of purified 17DD with two other strains of YF virus only fenome-sized molecules for all three viruses. These observations suggest that vaccine phenotype is primarily associated with the accumulation of mutations.

【 授权许可】

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