RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay

Memórias do Instituto Oswaldo Cruz

Marilda M. Siqueira¹  Vanja Ferreira¹  Jussara P. Nascimento¹ 

关键词: respiratory syncytial virus;    rapid diagnosis;    immunofluorescence;    enzyme immunoassay;   

DOI  :  10.1590/S0074-02761986000200013

来源: SciELO

【 摘 要 】

Two techniques for rapid diagnosis, immunofluorescence (IFAT) and enzyme immunoassay (EIA), have been comparedwith virus isolaion in tissue culturefor the detection of respiratory syncytial virus (RSV) in specimens of nasopharyngeal secretions. The specimens were obtained from children under five years of age suffering from acute respiratory iliness, during a period of six months from January to June 1982. Of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) were positive by immunofluorescence. The bacterial contamination of inoculated tissue cultures unfortunately prevented the isolation of virus from many samples. Specimens from 216 children were tested to compare enzyme immunoassay and immunofluorescence. Of these 60 (27%) were positive by EIA and 121 (56%) were positive by IFAT. Our results suggest that the EIA technique although highly specific is rather insensitive. This may be because by the time these tests were done the originl nasopharyngeal secretions were considerably diluted and contained more mucus fragments than the call suspension used for IFAT. Of the three techniques, IFAT gives the best results although EIA may be useful where IFAT is not possible.

【 授权许可】

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