Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Tauana De Sousa Ferreira¹  Thaís Tâmara Castro Minuzzi-souza¹  Andrey José De Andrade¹  Thais Oliveira Coelho¹  Douglas De Almeida Rocha¹  Marcos Takashi Obara¹  Mariana Hecht¹  Nadjar Nitz¹  Rodrigo Gurgel-gonçalves¹ 

关键词: PCR;    Trypanosomatids;    Phlebotominae.;   

DOI  :  10.1590/0037-8682-0076-2015

来源: SciELO

【 摘 要 】

Abstract:INTRODUCTION : This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil.METHODS : Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing.RESULTS : PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively.CONCLUSIONS : Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.

【 授权许可】

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