Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Lívia Maria Do Amorim Costa¹  Maria Emília De Castro Kling Fleming¹  Geraldo Renato De Paula¹  Lenise Arneiro Teixeira¹  Pedro Juan José Mondino¹  Sílvia Susana Bona De Mondino¹  Cláudia Rezende De Vieira Mendonça-souza¹ 

关键词: Pseudomonas aeruginosa;    Antimicrobial resistance;    Metallo-β-lactamase;   

DOI  :  10.1590/0037-8682-0198-2014

来源: SciELO

【 摘 要 】

INTRODUCTION: Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime-resistant P. aeruginosa isolates from State of Sergipe, Brazil. METHODS: Metallo-β-lactamase was investigated using the disk approximation test and polymerase chain reaction (PCR). Genetic diversity was evaluated by pulsed-field gel electrophoresis (PFGE). RESULTS: A total of 48 (51.6%) isolates were resistant to ceftazidime. Six (12.2%) of these were positive for metallo-β-lactamase production. Only two (4.1%) of the ceftazidime-resistant isolates carried the bla SPM-1 gene. CONCLUSIONS: Production of metallo-β-lactamases was not the main mechanism of resistance to ceftazidime and carbapenems among P. aeruginosa strains in Sergipe, Brazil.

【 授权许可】

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