Comparative study of cultivation of feces in vermiculite or charcoal to obtain larvae of Strongyloides venezuelensis

Revista da Sociedade Brasileira de Medicina Tropical

Steveen Rios Ribeiro¹  Caroline Ohnesorge Maia¹  Fausto Edmundo Lima Pereira¹  Narcisa Imaculada Brant Moreira¹ 

关键词: Charcoal;    Strongyloides;    Vermiculite;   

DOI  :  10.1590/0037-8682-0019-2014

来源: SciELO

【 摘 要 】

IntroductionWe compared feces culturing in charcoal or vermiculite to obtain Strongyloides venezuelensis larvae.MethodsFeces (5g) from infected rats was mixed with vermiculite (10g) or coal (10g) in plastic cups and incubated at 28°C for 48h. Larvae were recovered using Baermann-Moraes method.ResultsSignificantly higher number of positive larval cultures were recovered from vermiculite than from charcoal (15/17 and 4/17, respectively; p < 0.001; 990.6 ± 307.5 and 215 ± 78.1 larvae, p = 0.027).ConclusionsVermiculite yields more larvae and provides cleaner pellets, improving larvae identification and facilitating their use for other purposes.

【 授权许可】

CC BY   
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