High similarity of Trypanosoma cruzikDNA genetic profiles detected by LSSP-PCR within family groups in an endemic area of Chagas disease in Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Sandra Maria Alkmim-oliveira¹  Henrique Borges Kappel¹  Cristiane Pontes Andrade¹  Aluízio Prata¹  Luis Eduardo Ramirez¹  Dalmo Correia¹  Eliane Lages-silva¹ 

关键词: Trypanosoma cruzi;    Endemic area;    LSSP-PCR;   

DOI  :  10.1590/0037-8682-0255-2013

来源: SciELO

【 摘 要 】

IntroductionDetermining the genetic similarities among Trypanosoma cruzi populations isolated from different hosts and vectors is very important to clarify the epidemiology of Chagas disease.MethodsAn epidemiological study was conducted in a Brazilian endemic area for Chagas disease, including 76 chronic chagasic individuals (96.1% with an indeterminate form; 46.1% with positive hemoculture).ResultsT. cruzi I (TcI) was isolated from one child and TcII was found in the remaining (97.1%) subjects. Low-stringency single-specific-primer-polymerase chain reaction (LSSP-PCR) showed high heterogeneity among TcII populations (46% of shared bands); however, high similarities (80-100%) among pairs of mothers/children, siblings, or cousins were detected.ConclusionsLSSP-PCR showed potential for identifying similar parasite populations among individuals with close kinship in epidemiological studies of Chagas disease.

【 授权许可】

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