Improvement of a PCR test to diagnose infection by Mansonella ozzardi

Revista da Sociedade Brasileira de Medicina Tropical

Luana Janaína Souza Vera²  Sergio De Almeida Basano²  Juliana De Souza Almeida Aranha Camargo²  Andonai Krauze De França¹  Ricardo De Godoi Mattos Ferreira¹  Almeida Andrade Casseb¹  Jansen Fernandes Medeiros¹  Gilberto Fontes¹  Luís Marcelo Aranha Camargo² 

[1] ,Faculdade São Lucas Coordenação de Medicina Porto Velho RO

关键词: Mansonella ozzardi;    Diagnosis;    PCR;    Mansonella ozzardi;    Diagnóstico;    PCR;   

DOI  :  10.1590/S0037-86822011000300023

来源: SciELO

【 摘 要 】

INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.

【 授权许可】

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