Standardization of an ELISA test using a recombinant nucleoprotein from the Junin virus as the antigen and serological screening for arenavirus among the population of Nova Xavantina, State of Mato Grosso

Revista da Sociedade Brasileira de Medicina Tropical

Alex Martins Machado²  Glauciane Garcia Figueiredo²  Gelse Maria Campos²  Mario Enrique Lozano¹  Aline Rafaela Da Silva Rodrigues Machado²  Luiz Tadeu Moraes Figueiredo² 

[1] ,University of São Paulo in Ribeirão Preto School of Medicine Virology Research Center SP

关键词: Arenavirus hemorrhagic fever;    ELISA;    Junin;    Arenavirus in Brazil;    Arena virosis;    Febre hemorrágica por arenavírus;    ELISA;    Junin;    Arenavirus no Brasil;    Arenavirose;   

DOI  :  10.1590/S0037-86822010000300003

来源: SciELO

【 摘 要 】

INTRODUCTION: Arenavirus hemorrhagic fever is a severe emerging disease. METHODS: Considering that the levels of antibodies against arenavirus in the Brazilian population are completely unknown, we have standardized an ELISA test for detecting IgG antibodies using a recombinant nucleoprotein from the Junin virus as the antigen. This protein was obtained by inserting the gene of the Junin virus nucleoprotein into the genome of Autographa californica nucleopolyhedrovirus, using the Bac-to-Bac baculovirus expression system. This recombinant baculovirus was used to infect S. frugiperda cells (SF9). RESULTS: The infection resulted in synthesis of high concentrations of recombinant protein. This protein was detected on 12.5% polyacrylamide gel and by means of Western blot. Using the standardized ELISA test, 343 samples from the population of Nova Xavantina were analyzed. We observed that 1.4% of the serum samples (five samples) presented antibody titers against arenavirus. CONCLUSIONS: These results show the population studied may present exposure to arenavirus infection.

【 授权许可】

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