Comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods

Revista da Sociedade Brasileira de Medicina Tropical

Crystiane Rodrigues Araújo Mota¹  Karla Carvalho Miranda¹  Janine De Aquino Lemos¹  Carolina Rodrigues Costa¹  Lúcia Kioko Hasimoto E Souza¹  Xisto Sena Passos¹  Hildene Meneses E Silva¹  Maria Do Rosário Rodrigues Silva¹ 

[1] ,Federal University of Goiás Tropical Pathology and Public Health Institute Goiânia GO ,Brazil

关键词: In vitro susceptibility;    Agar dilution;    Broth microdilution;    Dermatophytes;    Suscetibilidade in vitro;    Diluição em ágar;    Microdiluição em caldo;    Dermatófitos;   

DOI  :  10.1590/S0037-86822009000300003

来源: SciELO

【 摘 要 】

The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. The percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, 88.3% for itraconazole, 81.6% for terbinafine and 73.3% for fluconazole. One hundred percent agreement was obtained for Trichophyton mentagrophytes isolates evaluated with ketoconazole and griseofulvin. Thus, until a reference method for testing the in vitro susceptibility of dermatophytes is standardized, the similarity of the results between the two methods means that the agar dilution method may be useful for susceptibility testing on these filamentous fungi.

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