Revista da Sociedade Brasileira de Medicina Tropical | |
Polymerase chain reaction and restriction fragment length polymorphism analysis of the ITS2 region for differatiation of Brazilian Biomphalaria intermediate hosts of the Schistosoma mansoni | |
Teofânia Heloísa Dutra Amorim Vidigal1  Kelly Grace Magalhães1  Omar Dos Santos Carvalho1  | |
[1] ,Fundação Oswaldo Cruz Centro de Pesquisas René Rachou Belo Horizonte MG ,Brasil | |
关键词: Biomphalaria glabrata; Biomphalaria tenagophila; Biomphalaria straminea; Polymerase chain reaction; Internal transcribed spacer 2; Biomphalaria glabrata; Biomphalaria tenagophila; Biomphalaria straminea; Reação em cadeia da polimerase; Região espaçadora transcrita interna 2; | |
DOI : 10.1590/S0037-86822004000400012 | |
来源: SciELO | |
【 摘 要 】
We sequenced the internal transcribed spacer 2 of the ribosomal DNA (ITS2-DNAr) from the three Schistosoma mansoni intermediate hosts in Brazil: Biomphalaria glabrata, Biomphalaria tenagophila and Biomphalaria straminea. Analysis of a restriction map from those sequences allowed us to select putative restriction enzymes able to identify the snail species under study. Four restriction enzymes were used and HpaII provided simple species-specific profiles easily visualized in polyacrylamide gels. The use of ITS2 is advantageous as it provides a small fragment of 460 bp which may be easily amplified by PCR. In the current work, we showed that the amplification of ITS2-DNAr together with HpaII enzyme restriction is an auxiliary molecular tool for the morphological identification of such snails as well as for taxonomic and phylogenetic studies of neotropical planorbids.
【 授权许可】
CC BY
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