Characterization of Vibrio Parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil

Revista do Instituto de Medicina Tropical de São Paulo

Martha Virginia Ribeiro Rojas¹  Maria Helena Matté¹  Milena Dropa¹  Miriam Lopes Da Silva¹  Glavur Rogério Matté¹ 

[1] ,University of São Paulo School of Public Health Public Health Laboratory

关键词: Vibrio parahaemolyticus;    tlh;    tdh and trh Genes;    blaTEM-116;   

DOI  :  10.1590/S0036-46652011000400005

来源: SciELO

【 摘 要 】

Vibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. Most of the clinical isolates produce thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) encoded by tdh and trh genes respectively. In this study, twenty-three V. parahaemolyticus, previously isolated from oysters and mussels were analyzed by PCR using specific primers for the 16S rRNA and virulence genes (tdh, trh and tlh) and for resistance to different classes of antibiotics and PFGE. Nineteen isolates were confirmed by PCR as V. parahaemolyticus. The tlh gene was present in 100% of isolates, the tdh gene was identified in two (10.5%) isolates, whereas the gene trh was not detected. Each isolate was resistant to at least one of the nine antimicrobials tested. Additionally, all isolates possessed the blaTEM-116 gene. The presence of this gene in V. parahaemolyticus indicates the possibility of spreading this gene in the environment. Atypical strains of V. parahaemolyticus were also detected in this study.

【 授权许可】

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