Histopathology, serology and cultures in the diagnosis of cryptococcosis

Revista do Instituto de Medicina Tropical de São Paulo

Alexandra Flávia Gazzoni¹  Cecília Bittencourt Severo¹  Emily Ferreira Salles¹  Luiz Carlos Severo² 

[1] ,Hospital Santa Rita Laboratório de Micologia Santa Casa-Complexo HospitalarPorto Alegre RS ,Brasil

关键词: Budding index;    Carminophilic index;    Mayer's mucicarmine stain;    Fontana-Masson stain;    Cryptococcal antigen test;    Cultures;   

DOI  :  10.1590/S0036-46652009000500004

来源: SciELO

【 摘 要 】

Cryptococcosis is one of the most common opportunistic fungal infections in patients with acquired immunodeficiency syndrome (AIDS). We report 13 cases of cryptococcal infection based on histopathology, serology and cultures. Epidemiological analysis, histochemical techniques of hematoxilin and eosin (HE) and Grocot's silver (GMS), as well special histochemical techniques such as Mayer's mucicarmine (MM) and Fontana-Masson (FM), cryptococcal antigen test (CrAg) and isolation on fungal media: Sabouraud's (SAB), brain-heart infusion agar (BHI) and canavanine-glycine-bromothymol blue (CGB) agar were analyzed. Unsatisfactory staining results by MM stain associated to negative titers by CrAg test, which FM stain confirmed that capsule-deficient Cryptococcus infections were observed in four cases. Eight isolated cases were identified as follows: six cases were infection with Cryptococcus neoformans and two cases were Cryptococcus gattii.

【 授权许可】

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