Revista do Instituto de Medicina Tropical de São Paulo | |
Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil | |
Milena Dropa2  Livia C. Balsalobre2  Nilton Lincopan1  Elsa M. Mamizuka1  Thays Murakami1  Valéria C. Cassettari1  Fábio Franco1  Stella M. Guida1  Angelica J. Balabakis1  Lilian F. Passadore1  Silvia R. Santos1  Glavur R. Matté2  Maria H. Matté2  | |
[1] ,University of São Paulo School of Public Health Public Health LaboratorySão Paulo SP ,Brazil | |
关键词: ESBL; Enterobacteriaceae; Klebsiella pneumoniae; Pulsed Field Gel Electrophoresis; Antimicrobial resistance; | |
DOI : 10.1590/S0036-46652009000400005 | |
来源: SciELO | |
【 摘 要 】
Extended-spectrum β-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for blaSHV, blaTEM and blaCTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. BlaSHV, blaTEM and blaCTX-M were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field gel eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, suggesting clonal spread in the institutional environment.
【 授权许可】
CC BY-NC
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