Classic and molecular study of Giardia duodenalis in children from a daycare center in the region of Presidente Prudente, São Paulo, Brazil

Revista do Instituto de Medicina Tropical de São Paulo

Nair Toshiko Tashima²  Maria Jacira Silva Simões¹  Clarice Queico Fujimura Leite¹  Antonio Fluminhan¹  Marco Antonio Nogueira¹  Ana Carolina Malaspina¹ 

[1] ,Universidade do Oeste Paulista Departamento de Parasitologia Presidente Prudente SP ,Brasil

关键词: Giardia duodenalis;    Enteroparasitosis;    Daycare center;    Children;    RAPD;    Epidemiology;   

DOI  :  10.1590/S0036-46652009000100004

来源: SciELO

【 摘 要 】

Epidemiological studies on giardiasis by using molecular techniques such as RAPD (Randomly Amplified Polymorphic DNA) may give information on factors related to the transmission of Giardia duodenalis. The aim of this work was to assess the epidemiology of G. duodenalis in 101 children attended at a daycare center in Presidente Bernardes, SP, Brazil. After parasitological examinations in feces samples, 15 children presented cysts of G. duodenalis. Their respective parents, brothers and pets, besides the daycare center workers, also had their feces submitted to parasitological analysis. Seven mothers and nine brothers also presented G. duodenalis cysts, while fathers, daycare workers and pets (dogs) did not presented the parasite. Besides the 15 cases with G. duodenalis, other 23 children presented other enteroparasites (Entamoeba coli, Endolimax nana, Enterobius vermicularis, Ascaris lumbricoides and Trichuris trichiura). Samples of G. duodenalis cysts from children and their relatives were submitted to molecular typing by RAPD after genomic DNA extraction and amplification of a fragment of the 18S rDNA region by PCR. After examining 31 isolates of G. duodenalis (children and their respective mothers and brothers), it was concluded that the parasite transmission occurred in children, probably during daily cohabitation at the daycare center, but not at home among their relatives or pets.

【 授权许可】

CC BY-NC   
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