Performances of HTLV serological tests in diagnosing HTLV infection in high-risk population of São Paulo, Brazil

Revista do Instituto de Medicina Tropical de São Paulo

Fabrício Jacob²  Elizabeth De Los Santos-fortuna²  Raymundo Soares Azevedo¹  Adele Caterino-de-araujo² 

[1] ,Instituto Adolfo Lutz Seção de Imunologia São Paulo SP ,Brasil

关键词: Human T-lymphotropic virus type I and II (HTLV-I and HTLV-II);    Serology;    Enzyme immunoassay (EIA);    Diagnosis;    Performance;    High-risk population;   

DOI  :  10.1590/S0036-46652007000600005

来源: SciELO

【 摘 要 】

Testing problems in diagnosing human T-lymphotropic virus (HTLV) infection, mostly HTLV-II, have been documented in HIV/AIDS patients. Since December 1998, the Immunology Department of Instituto Adolfo Lutz (IAL) offers HTLV-I/II serology to Public Health Units that attend HTLV high-risk individuals. Two thousand, three hundred and twelve serum samples: 1,393 from AIDS Reference Centers (Group I), and 919 from HTLV out-patient clinics (Group II) were sent to IAL for HTLV-I/II antibodies detection. The majority of them were screened by two enzyme immunoassays (EIAs), and confirmed by Western Blot (WB 2.4, Genelabs). Seven different EIA kits were employed during the period, and according to WB results, the best performance was obtained by EIAs that contain HTLV-I and HTLV-II viral lysates and rgp21 as antigens. Neither 1st and 2nd, nor 3rd generation EIA kits were 100% sensitive in detecting truly HTLV-I/II reactive samples. HTLV-I and HTLV-II prevalence rates of 3.3% and 2.5% were detected in Group I, and of 9.6% and 3.6% in Group II, respectively. High percentages of HTLV-seroindeterminate WB sera were detected in both Groups. The algorithm testing to be employed in HTLV high-risk population from São Paulo, Brazil, needs the use of two EIA kits of different formats and compounds as screening, and because of high seroindeterminate WB, may be another confirmatory assay.

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