Occurrence of Proteus mirabilis associated with two species of venezuelan oysters

Revista do Instituto de Medicina Tropical de São Paulo

Milagro Fernández-delgado²  Monica Contreras¹  María Alexandra García-amado¹  Pulchérie Gueneau¹  Paula Suárez² 

[1] ,Universidad Simón Bolívar Departamento de Biología de Organismos Caracas,Venezuela

关键词: Proteus mirabilis in bivalves molluscan;    Isognomon alatus;    Crassostrea rhizophorae;    Venezuelan coastal area;    Public health and environmental impact;    Antibiotic resistance;   

DOI  :  10.1590/S0036-46652007000600004

来源: SciELO

【 摘 要 】

The fecal contamination of raw seafood by indicators and opportunistic pathogenic microorganisms represents a public health concern. The objective of this study was to investigate the presence of enteric bacteria colonizing oysters collected from a Venezuelan touristic area. Oyster samples were collected at the northwestern coast of Venezuela and local salinity, pH, temperature, and dissolved oxygen of seawater were recorded. Total and fecal coliforms were measured for the assessment of the microbiological quality of water and oysters, using the Multiple Tube Fermentation technique. Analyses were made using cultures and 16S rRNA gene sequencing. Diverse enrichment and selective culture methods were used to isolate enteric bacteria. We obtained pure cultures of Gram-negative straight rods with fimbriae from Isognomon alatus and Crassostrea rhizophorae. Our results show that P. mirabilis was predominant under our culture conditions. We confirmed the identity of the cultures by biochemical tests, 16S rRNA gene sequencing, and data analysis. Other enterobacteria such as Escherichia coli, Morganella morganii and Klebsiella pneumoniae were also isolated from seawater and oysters. The presence of pathogenic bacteria in oysters could have serious epidemiological implications and a potential human health risk associated with consumption of raw seafood.

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