Inhibitory effect of deferoxamine on Paracoccidioides brasiliensis survival in human monocytes: reversal by holotransferrin not by apotransferrin

Revista do Instituto de Medicina Tropical de São Paulo

Luciane Alarcão Dias-melicio²  Sueli Aparecida Calvi¹  Maria Terezinha Serrão Peraçoli²  Ângela Maria Victoriano De Campos Soares² 

[1] ,São Paulo State University Biosciences Institute Department of Microbiology and ImmunologyBotucatu SP ,Brazil

关键词: Paracoccidioides brasiliensis;    Monocytes;    Iron;    Deferoxamine;   

DOI  :  10.1590/S0036-46652005000500005

来源: SciELO

【 摘 要 】

The mechanisms used by Paracoccidioides brasiliensis to survive into phagocytic cells are not clear. Cellular iron metabolism is of critical importance to the growth of several intracellular pathogens whose capacity to multiply in mononuclear phagocytes is dependent on the availability of intracellular iron. Thus, the objective of this paper was to investigate the role of intracellular iron in regulating the capacity of P. brasiliensis yeast cells to survive within human monocytes. Treatment of monocytes with deferoxamine, an iron chelator, suppressed the survival of yeasts in a concentration-dependent manner. The effect of deferoxamine was reversed by iron-saturated transferrin (holotransferrin) but not by nonsaturated transferrin (apotransferrin). These results strongly suggest that P. brasiliensis survival in human monocytes is iron dependent.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License