Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (Viannia) subgenus

Revista do Instituto de Medicina Tropical de São Paulo

Byanca Regina Paiva²  Luciana Neves Passos¹  Aloisio Falqueto¹  Rosely Dos S. Malafronte²  Heitor Franco De Andrade Jr.² 

[1] ,Universidade de São Paulo Instituto de Medicina Tropical de São Paulo São Paulo SP ,Brasil

关键词: Leishmaniasis (Viannia);    PCR;    Identification;   

DOI  :  10.1590/S0036-46652004000600007

来源: SciELO

【 摘 要 】

In Brazil, the main etiologic agent of Leishmaniasis that frequently presents with mucosal involvement belongs to the Viannia subgenus. The therapeutic conduct in this disease depends on the parasitological diagnosis, and classical methods are restricted in identifying the agent. In this paper we describe a polymerase chain reaction (PCR), which uses primers designed from mini-exons repetitive sequences. The PCR amplifies a 177bp fragment that can distinguish (Viannia) from (Leishmania) subgenus. This test could be a useful diagnostic tool.

【 授权许可】

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