PCR - based diagnosis to evaluate the performance of malaria reference centers

Revista do Instituto de Medicina Tropical de São Paulo

Silvia Maria Di Santi²  Karin Kirchgatter²  Karen Cristina Sant'anna Brunialti²  Alessandra Mota Oliveira²  Sergio Roberto Santos Ferreira²  Marcos Boulos¹ 

[1] ,Universidade de São Paulo Instituto de Medicina Tropical de São Paulo Superintendência de Controle de Endemias

关键词: Malaria;    Diagnosis;    Plasmodium;    PCR;    ssrRNA;    Thick blood smear;   

DOI  :  10.1590/S0036-46652004000400002

来源: SciELO

【 摘 要 】

Although the Giemsa-stained thick blood smear (GTS) remains the gold standard for the diagnosis of malaria, molecular methods are more sensitive and specific to detect parasites and can be used at reference centers to evaluate the performance of microscopy. The description of the Plasmodium falciparum, P. vivax, P. malariae and P. ovale ssrRNA gene sequences allowed the development of a polymerase chain reaction (PCR) that had been used to differentiate the four species. The objective of this study was to determine Plasmodium species through PCR in 190 positive smears from patients in order to verify the quality of diagnosis at SUCEN's Malaria Laboratory. Considering only the 131 positive results in both techniques, GTS detected 4.6% of mixed and 3.1% of P. malariae infections whereas PCR identified 19.1% and 13.8%, respectively.

【 授权许可】

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