"Multiplex PCR" identification of the atypical and monophasic Salmonella enterica subsp. enterica serotype 1,4,[5],12:i:- in São Paulo State, Brazil: frequency and antibiotic resistance patterns

Revista do Instituto de Medicina Tropical de São Paulo

Ana T. Tavechio¹  Ângela C. R. Ghilardi¹  Sueli A. Fernandes¹ 

[1] ,Instituto Adolfo Lutz Enteropathogens Laboratory São Paulo,Brazil

关键词: Salmonella;    Serotyping;    Antimicrobial resistance;    PCR analysis;   

DOI  :  10.1590/S0036-46652004000200012

来源: SciELO

【 摘 要 】

Salmonella spp. are the etiologic agents of salmonellosis, a worldwide spread zoonoses causing foodborne outbreaks and clinical diseases. By serological identification, Salmonella enterica subsp. enterica serotype 1,4,[5],12:i:- accounted for 8.8% of human and 1.6% of nonhuman Salmonella strains isolated in São Paulo State, during 1991-2000. A total of 28.6% of them amplified a fragment corresponding to H:1,2 (flagellar phase two) through PCR analysis and were further assigned as S. Typhimurium. Antimicrobial resistance was detected in 36.3% of the 369 PCR-negative strains tested, including the multiresistance to ampicillin, chloramphenicol, sulfonamides, tetracycline, and streptomycin.

【 授权许可】

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