Occurrence of Cryptosporidium oocysts and Giardia cysts in raw water from the Atibaia river, Campinas, Brazil

Revista do Instituto de Medicina Tropical de São Paulo

Regina Maura Bueno Franco²  Rosângela Rocha-eberhardt²  Romeu Cantusio Neto¹ 

[1] ,State University of Campinas Institute of Biology Department of ParasitologyCampinas SP ,Brazil

关键词: Cryptosporidium;    Giardia;    Raw water;    Brazil;   

DOI  :  10.1590/S0036-46652001000200011

来源: SciELO

【 摘 要 】

Cryptosporidium parvum and Giardia duodenalis are waterborne parasites that have caused several outbreaks of gastrointestinal disease associated with drinking water. Due to the lack of studies about the occurrence of these protozoa in water in the Southeast of Brazil, an investigation was conducted to verify the presence of cysts and oocysts in superficial raw water of the Atibaia River. The water samples were submitted to membrane filtration (3.0 mum) and elution was processed by (1) scraping and rinsing of membrane (RM method) and (2) acetone-dissolution (ADM method). Microbiologic and chemical parameters were analyzed. Aliquots of the pellets were examined by immunofluorescence (Merifluor, Meridian Diagnostics, Cincinnati, Ohio). All water samples were positive for Cryptosporidium and Giardia, in spite of the high turbidity. Higher recovery rates occurred in samples treated by the RM method than by the ADM technique. The goal for future work is the assessment of viability of cysts and oocysts to determine the public health significance of this finding.

【 授权许可】

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