Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens

Revista do Instituto de Medicina Tropical de São Paulo

Noeli M. EspÍndola¹  Elizabeth N. De Gaspari²  Paulo M. Nakamura²  Adelaide J. Vaz¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas São Paulo SP ,Brasil

关键词: Taenia solium;    Taenia crassiceps;    Monoclonal antibody;    Cysticercosis;   

DOI  :  10.1590/S0036-46652000000300013

来源: SciELO

【 摘 要 】

We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra specific IgG-clones, 5 of them also recognizing Tso antigens. Two clones identified the 8-14 and 18kD peptides of VF-Tcra.

【 授权许可】

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