Diagnosis of human toxoplasmosis by a dot enzyme-linked immunosorbent assay

Revista do Instituto de Medicina Tropical de São Paulo

Mohamed Mohamed Anwar Elsaid²  Maria Terezinha Bahia¹  George Luiz Machado-coelho¹  Ricardo Wagner De Almeida Vitor² 

[1] ,UFMG ICB Departamento de ParasitologiaBelo Horizonte MG ,Brasil

关键词: Human toxoplasmosis;    Dot-ELISA;    Serodiagnosis;   

DOI  :  10.1590/S0036-46651995000200005

来源: SciELO

【 摘 要 】

A Dot enzyme-linked immunosorbent assay (Dot-ELISA) was standardized and evaluated for the serodiagnosis of human toxoplasmosis. Out of 538 serum samples tested by the immunofluorescence test for toxoplasmosis (IFAT-IgG) as reference test, 183 (34%) were positive at cut off 1:16 and 192 (36%) were positive for Dot-ELISA-IgG at cut-off 1:256. For Dot-ELISA, co-positivity was 0.94, co-negativity 0.94 and concordance 0.88 in relation to IFAT-IgG. These results suggest the usefulness of Dot-ELISA (cut-off titer of 1:256) for the serodiagnosis of human toxoplasmosis. The main advantage of this technique is simplicity, positive test can be visually identified (colored precipitate). It does not require a special equipment and it can be used as a qualitative test to screen large numbers of samples or as a quantitative assay to determine end-point titration of individual sera.

【 授权许可】

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