Evaluation of enzyme-linked immunosorbent and alternative assays for detection of HIV antibodies using panels of Brasilian sera

Revista do Instituto de Medicina Tropical de São Paulo

Jairo Ivo-dos-santos²  Deise L. Campos Mello¹  José C. Couto-fernandez²  Roberto M. Passos¹  Leila A. Dias-carneiro¹  Euclides A. Castilho¹  Bernardo Galvão-castro² 

[1] ,Fundação Oswaldo Cruz WHO Collaborating Centre on AIDS Salvador Bahia ,Brasil

关键词: Quality control;    HIV antibody tests;    Brazilian sera;   

DOI  :  10.1590/S0036-46651990000200006

来源: SciELO

【 摘 要 】

Sera from 472 Brazilian subjects, confirmed to be either positive or negative for HIV antibodies and comprising the total clinical spectrum of HIV infection, were utilized in the evaluation of six commercially available enzyme-linked immunosorbent assays (ELISA), as well as of four alternative assays, namely indirect immunofluorescence (IIF), passive hemagglutination (PHA), dot blot and Karpas AIDS cell test. The sensitivities ranged from 100% (Abbott and Roche ELISA) to 84.2% (PHA) and the specificities ranged from 99.3% (IIF) to 80.2% (PHA). The sensitivity and specificity of the PHA and the sensitivity of the Karpas cell test were significantly lower than those of the other tests. Although the IFF and dot blot had good sensitivities and specificities, the six ELISA were more attractive than those tests when other parameters such as ease of reading and duration of assay were considered.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License