Differences between in vitro and in vivo obtained schistosomules

Revista do Instituto de Medicina Tropical de São Paulo

Alan L. Melo¹  Conceição R. S. Machado²  Leógenes H. Pereira¹ 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de ParasitologiaBelo Horizonte MG ,Brasil

关键词: Schistosoma mansoni;    transformation;    Schistosomula;    in vivo;    in vitro;   

DOI  :  10.1590/S0036-46651990000200003

来源: SciELO

【 摘 要 】

The injection of cercariae of Schistosoma mansoni into the peritoneal cavity of naive mice induces cell adhesion to these larvae, and this adherence sharply decreases when the infecting larva changes to schistosomule. This procedure was used to detect differences between schistosomules obtained in vivo and in vitro. Reinoculation of schistosomules obtained in vivo into the peritoneal cavity of mice did not trigger cell adhesion. In contrast, adherent cells were found in 4 and 24-hour-in vitro schistosomules. Our data on schistosomules obtained in vitro indicate that more than 24 hours are needed for complete remotion of molecules involved in the phenomenon of cell adhesion.

【 授权许可】

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