Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi

Revista do Instituto de Medicina Tropical de São Paulo

N. Yoshida¹  M. M. G. Teixeira¹  C. A. Sbravate¹ 

[1] ,Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e Parasitologia São Paulo SP ,Brasil

关键词: Chagas'disease;    vector borne;    Trypanosoma cruzi;    cultured metacyclic tripomastigotes;   

DOI  :  10.1590/S0036-46651986000200003

来源: SciELO

【 摘 要 】

Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein in both types of CL strain metacyclics. A monoclonal antibody raised against G strain metacyclics, and specific for metacyclic stages of this strain, reacted with both CL strain metacyclic types. Both metacyclic forms were similarly Iysed by various anti-T. cruzi sera, in a complement-mediated reaction.

【 授权许可】

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