RC-IAL cell line: sensitivity of rubella virus grow

Revista de Saúde Pública

Cristina A Figueiredo¹  Maria I Oliveira¹  Suely P Curti¹  Aurea S Cruz¹  Eliane Moreira¹  Ana Ms Afonso¹  Luís Florêncio De Salles-gomes¹ 

[1] ,Instituto Adolfo Lutz Serviço de Virologia São Paulo SP ,Brasil

关键词: Rubella virus;    Cell line;    Cytopathogenic effect;    viral;    Virus replication;    Epithelial cells;    Rubella;    Vírus da rubéola;    Linhagem celular;    Efeito citopatogênico viral;    Replicação viral;    Células epiteliais;    Rubéola (sarampo alemão);   

DOI  :  10.1590/S0034-89102000000400007

来源: SciELO

【 摘 要 】

OBJECTIVE: The rapid growth of the rubella virus in RC-IAL² with development of cytopathic effect, in response to rubella virus infection, is described. For purposes of comparison, the rubella virus RA-27/3 strain was titered simultaneously in the RC-IAL, Vero, SIRC and RK13 cell lines. METHODS: Rubella virus RA-27/3 strain are inoculated in the RC-IAL cell line (rabbit Kidney, Institute Adolfo Lutz). Plates containing 1.5x10(5) cells/ml of RC-IAL line were inoculated with 0.1ml s RA-27/3 strain virus containing 1x 10(4)TCID50/0.1ml. A 25% cytopathic effect was observed after 48 hours and 100% after 96 hours. The results obtained were compared to those observed with the SIRC, Vero and RK13 cell lines. Rubella virus was detected by immunohistochemistry. RESULTS: With the results, it was possible to conclude that the RC-IAL cell line is a very good substrate for culturing rubella virus. The cells inoculated with rubella virus were examined by phase contrast microscopy and showed the characteristic rounded, bipolar and multipolar cells. The CPE in RC-IAL was observed in the first 48 hours and the curve of the increased infectivity was practically the same as observed in other cell lines. CONCLUSIONS: These findings are important since this is one the few cell lines described in the literature with a cytopathic effect. So it can be used for antigen preparation and serological testing for the diagnosis of specific rubella antibodies.

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