Chemical sterilization in in vitro propagation of Arundina bambusifolia Lindl. and Epidendrum ibaguense Kunth

Revista Ceres

Donizetti Tomaz Rodrigues²  Roberto Ferreira Novais¹  Víctor Hugo Alvarez Venegas¹  José Maria Moreira Dias¹  Wagner Campos Otoni¹  Ecila Mercês De Albuquerque Villani¹ 

[1],Universidade Federal de Viçosa Departamento de Solos Viçosa Minas Gerais ,Brazil

关键词: tissue culture;    contamination;    sodium hyplochlorite;    orchid;    cultura de tecido;    contaminação;    hipoclorito de sódio;    orquídea;   

DOI  :  10.1590/S0034-737X2013000400001

来源: SciELO

【 摘 要 】

There is a great demand for simpler and less costly laboratory techniques and for more accessible procedures for orchid breeders who do not have the necessary theoretical basis to use the traditional seed and clone production methods of orchids in vitro. The aim of this study was to assess the use of sodium hypochlorite (NaClO) as a decontaminant in the process of inoculating adult orchid explants of Arundina bambusifolia and Epidendrum ibaguenses. Solutions of NaClO (1.200, 2.400, 3.600, 4.800 and 6.000 mg L-1 - equivalent to 50, 100, 150, 200 and 250 mL L-1 of commercial bleach - CB) were sprayed on the explants (1.0 mL) and the culture medium (GB5), in the presence or absence of activated charcoal (2 g L-1). The explants used were nodal segments of field-grown adult plants. The procedures for inoculating the explants were conducted outside the laminar flow chamber (LFC), except for the control treatment (autoclaved medium and explant inoculation inside the LFC). The best results for fresh weight yield, height and number of shoots were obtained using NaClO in solution at 1.200 mg L-1 (equivalent to 50 mL L-1 commercial bleach) with activated charcoal in the culture medium. Fresh weight figures were 1.10 g/jar for Arundina bambusifolia and 0.16 g/jar for Epidendrum ibaguenses. Spraying the NaClO solutions controls the contamination of the culture medium already inoculated with the explants.

【 授权许可】

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