Genetic diversity in sugar apple (Annona squamosa L.) by using RAPD markers

Revista Ceres

João Filipi Rodrigues Guimarães²  Silvia Nietsche¹  Márcia Regina Costa¹  Glaucia Bethania Rocha Moreira¹  Marlon Cristian Toledo Pereira¹  Wagner Vendrame¹ 

[1] ,Universidade Federal Rural de Pernambuco Departamento de Agronomia Recife Pernambuco ,Brasil

关键词: accessions;    molecular marker;    variability;    germplasm;    acessos;    marcador molecular;    variabilidade;    germoplasma;   

DOI  :  10.1590/S0034-737X2013000300017

来源: SciELO

【 摘 要 】

Genetic diversity in a collection of 64 sugar apple accessions collected from different municipalities in northern Minas Gerais was assessed by RAPD analysis. Using 20 selected RAPD primers 167 fragments were generated, of which 48 were polymorphic (28.7%) producing an average of 2.4 polymorphic fragments per primer. Low percentage of polymorphism (< 29%) was observed by using the set of primers indicating low level of genetic variation among the 64 accessions evaluated. Genetic relationships were estimated using Jaccard's coefficient of similarity. Accessions from different municipalities clustered together indicating no correlation between molecular grouping and geographical origin. The dendrogram revealed five clusters. The first cluster grouped C19 and G29 accessions collected from the municipalities of Verdelândia and Monte Azul, respectively. The second cluster grouped G16 and B11 accessions collected from the municipalities of Monte Azul and Coração de Jesus, respectively. The remaining accessions were grouped in three clusters, with 8, 15 and 37 accessions, respectively. In summary, RAPD showed a low percentage of polymorphism in the germplasm collection.

【 授权许可】

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