The influence of light spectra, UV-A, and growth regulators on the in vitro seed germination of Senecio cineraria DC.

Revista Ceres

Cristiane Pimentel Victório²  Nina Cláudia Barbosa Da Silva¹  Maria Apparecida Esquibel¹  Alice Sato¹ 

[1] ,Universidade Federal do Rio de Janeiro Instituto de Biofísica Carlos Chagas Filho Rio de Janeiro Rio de Janeiro ,Brasil

关键词: Auxin;    gibberellin;    light spectra;    plant tissue cultures;    ultraviolet-A;    Auxina;    cultura de tecidos vegetais;    espectros de luz;    giberelina;    ultravioleta-A;   

DOI  :  10.1590/S0034-737X2010000500002

来源: SciELO

【 摘 要 】

This study was carried out to investigate the effects of light spectra, additional UV-A, and different growth regulators on the in vitro germination of Senecio cineraria DC. Seeds were surface-sterilized and inoculated in MS medium to evaluate the following light spectra: white, white plus UV-A, blue, green, red or darkness. The maximum germinability was obtained using MS0 medium under white light (30%) and MS + 0.3 mg L-1 GA3 in the absence of light (30.5%). S. cineraria seeds were indifferent to light. Blue and green lights inhibited germination. Different concentrations of gibberellic acid (GA3) (0.1; 0.4; 0.6; 0.8; 1.0 and 2.0 mg L-1) and indole-3-acetic acid IAA (0.1; 0.3 and 1.0 mg L-1) were evaluated under white light and darkness. No concentration of GA3 enhanced seed germination percentage under white light. However, when the seeds were maintained in darkness, GA3 improved germination responses in all tested concentrations, except at 1.0 mg L-1. Under white light, these concentrations also increased the germination time and reduced germination rate. Germination rate, under light or darkness, was lower using IAA compared with GA3.

【 授权许可】

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