Novel use of trypan blue in ocular surface staining: redefining implications for this vital dye

Revista Brasileira de Oftalmologia

Renato Ambrósio Jr.²  Habeeb Ahmad¹  Diogo Caldas¹  Ana Laura Caiado Canedo²  Bruno Valbon²  Frederico Procopio Guerra²  Acácio Alves De Souza Lima¹ 

[1] ,Instituto de Olhos Renato AmbrósioRio de Janeiro RJ ,Brazil

关键词: Eye diseases;    Ophthalmologic surgical procedures;    Trypan blue;    Staining and labeling;    Case reports;    Oftalmopatias;    Procedimentos cirúrgicos oftalmológicos;    Azul tripano;    Coloração e rotulagem;    Relatos de casos;   

DOI  :  10.1590/S0034-72802011000600014

来源: SciELO

【 摘 要 】

Different applications of trypan blue (TB) for intraocular surgery have been reported, with very high levels of safety and efficacy. We describe the use of TB as an alternative vital dye for staining the ocular surface to assess the integrity of superficial cell layers of the cornea and the surface environment. This facilitates the diagnosis of various ocular surface disorders, including screening for dry eye disease (DED) among refractive and cataract patients. TB staining properties are different from fluorescein and both are stable in a solution, so that a double staining technique is introduced.

【 授权许可】

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