A thioacetamide-induced hepatic encephalopathy model in C57BL/6 mice: a behavioral and neurochemical study

Arquivos de Neuro-Psiquiatria

Aline Silva De Miranda²  David Henrique Rodrigues²  Luciene Bruno Vieira¹  Cristiano Xavier Lima²  Milene Alvarenga Rachid²  Paula Vieira Teixeira Vidigal¹  Marcus Vinicius Gomez¹  Helton José Dos Reis¹  Cristina Guatimosim¹  Antônio Lúcio Teixeira² 

[1] ,Federal University of Minas Gerais Institute of Biological Sciences Department of Biochemistry and ImmunologyBelo Horizonte MG ,Brazil

关键词: hepatic encephalopathy;    thioacetamide;    behavioral changes;    SHIRPA;    glutamate;    encefalopatia hepática;    tioacetamida;    alterações comportamentais;    SHIRPA;    glutamato;   

DOI  :  10.1590/S0004-282X2010000400022

来源: SciELO

【 摘 要 】

OBJECTIVE: Hepatic encephalopathy (HE) is a neuropsychiatric syndrome resulting from liver failure. In the present study, we aimed to standardize an animal model of HE induced by thioacetamide (TAA) in C57BL/6 mice evaluating behavioral symptoms in association with liver damage and alterations in neurotransmitter release. METHOD: HE was induced by an intraperitoneal single dose of TAA (200 mg/kg, 600 mg/kg or 1,200 mg/kg). Behavioral symptoms were evaluated using the SHIRPA battery. Liver damage was confirmed by histopathological analysis. The glutamate release was measured using fluorimetric assay. RESULTS: The neuropsychiatric state, motor behavior and reflex and sensory functions were significantly altered in the group receiving 600 mg/kg of TAA. Biochemical analysis revealed an increase in the glutamate release in the cerebral cortex of HE mice. CONCLUSION: HE induced by 600mg/kg TAA injection in C57BL/6 mice seems to be a suitable model to investigate the pathogenesis and clinical disorders of HE.

【 授权许可】

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