Remyelination in experimentally demyelinated connexin 32 KnockOut mice

Arquivos de Neuro-Psiquiatria

Adriano Tony Ramos²  Paulo César Maiorka¹  Maria Lúcia Zaidan Dagli¹  Fernando Yutaka Moniwa Hosomi¹  Kalan Bastos Violin¹  Andréa Latorre¹  Aline De Marco Viott¹  Eduardo Kenji Masuda²  Maria Elisa Trost²  Tessie Beck Martins²  Dominguita Lühers Graça² 

[1] ,Federal University of Santa Maria Center of Health Sciences Departament of PathologySanta Maria RS ,Brazil

关键词: connexins;    myelin;    KnockOut mice;    conexinas;    mielina;    camundongos KnockOut;   

DOI  :  10.1590/S0004-282X2009000300020

来源: SciELO

【 摘 要 】

The aim of this study was to evaluate the role of connexin 32 (Cx 32) during remyelination of the peripheral nervous system, through a local injection of either 0,1% ethidium bromide solution or saline in the sciatic nerve of Cx 32 knockout mice. Euthanasia was performed ranging from 1, 2, 3, 7, 15, 21 to 30 days after injection. Histochemical, immunohistochemical, immunofluorescence and transmission electron microscopical techniques were used to analyze the development of the lesions. Within the sciatic nerves, Schwann cells initially showed signs of intoxication and rejected their sheaths; after seven days, some thin newly formed myelin sheaths with uneven compactness and redundant loops (tomacula) were conspicuous. We concluded that the regeneration of lost myelin sheaths within the PNS followed the pattern already reported for this model in other laboratory species. Therefore, these results suggest that absence of Cx 32 did not interfere with the normal pattern of remyelination in this model in young mice.

【 授权许可】

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