Mechanical hypernociception in experimental autoimmune encephalomyelitis

Arquivos de Neuro-Psiquiatria

David Henrique Rodrigues¹  Daniela Sachs¹  Antonio Lucio Teixeira¹ 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de Bioquímica e ImunologiaBelo Horizonte MG ,Brasil

关键词: multiple sclerosis;    experimental autoimmune encephalomyelitis;    hyperalgesia;    mechanical hypernociception;    esclerose múltipla;    encefalomielite autoimune experimental;    hiperalgesia;    hipernocicepção mecânica;   

DOI  :  10.1590/S0004-282X2009000100019

来源: SciELO

【 摘 要 】

BACKGROUND: Pain is an important clinical manifestation in multiple sclerosis (MS) patients, though it has been neglected in clinical and experimental researches. OBJECTIVE: To investigate the nociceptive response in MOG35-55 experimental autoimmune encephalomyelitis (EAE)-induced mice. METHOD: EAE was induced in 8 to 10 week old C57BL/6 female mice with an emulsion of MOG35-55, Complete Freund Adjuvant, Mycobacterium tuberculosis H37 RA and pertussis toxin. Nociception was evaluated by the von Frey filaments method. A clinical scale ranging from 0 to 15 was used to assess motor impairment. RESULTS: Clinical evidence of disease started at day 10 and peaked at day 14 after immunization. Thereafter, there was no worsening of symptoms until day 26. The EAE-induced mice presented reduced pressure threshold at days 7th and 10th after immunization and before the onset of clinical motor signs. CONCLUSION : The hypernociception found validates MOG35-55 EAE as a model for the study of pain in multiple sclerosis.

【 授权许可】

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